Tong Liu#, Yu-Gu Xie#, Feng Lin#, Li-Hua Xie#, Wen-Qiang Yang, Xing-Hua Su, Chun-Quan Ou, Lei Luo, Qi Xiao, Lu Gan and Xiao-Guang Chen*

ABSTRACT

Background: Aedes albopictus is the primary vector of mosquito-borne diseases, including dengue and chikungunya, in China. The management of vector mosquitoes is the primary strategy for the control of such infectious diseases. The gravid Ae. albopictus prefers to skip-oviposit its eggs into different small water containers, and the management of these breeding places is critical for mosquito control. Bacillus thuringiensis subspecies Israelensis (Bti) is a useful biological larvicide, but the effective period of the currently available commercial product is relatively short. This study aimed to develop a long-lasting formulation of Bti to control the dengue vector mosquito Ae. albopictus.

Results: Water-soluble polyethylene glycols and water-insoluble hexadecanol were mixed with Bti to develop the long-lasting formulation Bti-BLOCK, based on the solid dispersion technique. The controlled release of Bti-BLOCK and its effect on Ae. albopictus were assayed in the laboratory and in the field. The results showed that Bti toxins were slowly released from Bti-BLOCK into the water and maintained at an effective dose for at least 6 months. Bti-BLOCK caused high mortality during the immature stage (>90%) and achieved full inhibition during pupation (100%). The efficacy lasted at least 12 weeks in the laboratory and 6 weeks in the field. Furthermore, we confirmed an 89% reduction in Ae. albopictus density and a reduction in the R0 of dengue to a low-risk level after 6 months of open-field interventions.

Conclusions: We developed a long-lasting biological larvicide, Bti-BLOCK, which displayed very good efficacy in the control of the dengue vector mosquito Ae. albopictus.

Keywords: Aedes albopictus; Bacillus thuringiensis subspecies Israelensis; Larvicide; dengue.

Pest Manag Sci. 2021 Feb;77(2):741-748.

DOI : https://doi.org/10.1002/ps.6069